妖姬直播

May 2025

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  MS in Chemical Sciences Thesis Defense

  10:00am

  at Clendenin Building, CL 1010

  Speaker: Kaniz Fatama Khan, MSCB student
  Title: Biophysical and Biochemical Characterization of Flavone O-methyltransferase from Oryza sativa: Understanding and Engineering Enzymes to Enhance Flavonoid Biosynthesis in Plants
  Abstract: Methylated flavonoids exhibit enhanced biological activities compared to their unmethylated counterparts and function as antibiotics, antivirals, anti-cancer, anti-inflammatory, anti-obesity, neuroprotective, and antiallergic agents. During my time in the Master of Science in Chemical Sciences (MSCB) program at 妖姬直播 (KSU), I investigated the flavonoid biosynthetic pathway, focusing specifically on flavone 3'-O-methyltransferase (OsMT) from Oryza sativa (rice), an S-adenosyl methionine (SAM)-dependent methyltransferase involved in essential cellular processes, including the metabolism of xenobiotic and endogenous compounds. To elucidate the enzymatic function and potential applications of OsMT, we determined its three-dimensional (3D) structure and employed molecular biology and biochemical approaches to evaluate its activity and identify the roles of active site amino acid residues. Using QuikChange mutagenesis, I performed site-directed mutagenesis to generate point mutations targeting both the substrate and SAM cofactor binding sites. The contributions of these mutations to catalysis and binding were assessed through steady-state kinetics of wild-type and mutant enzymes, complemented by binding assays using nano differential scanning fluorimetry (nanoDSF) and microscale thermophoresis (MST). These kinetic and binding studies provided valuable insights into how specific mutations influence enzymatic activity and substrate specificity. Overall, this thesis work establishes a foundation for understanding key residues in OsMT function and contributes to our broader knowledge of flavone biosynthesis in rice. Furthermore, several OsMT mutants identified potential regions that can be engineered to alter enzyme characteristics, such as substrate specificity and catalytic efficiency. This approach has the potential to modify plant secondary metabolism to enhance resistance to pests or environmental stress and to enable the production of bioactive compounds with improved pharmacokinetic properties, such as anti-inflammatory and anti-cancer flavonoids.

   

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July 2025

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August 2025

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September 2025

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October 2025

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November 2025

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December 2025

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January 2026

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February 2026

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March 2026

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April 2026

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No events for this month